By Douglas T. Carrell, Peter N. Schlegel, Catherine Racowsky, Luca Gianaroli
Now in its fourth quantity, the Biennial evaluate of Infertility brings jointly the main updated examine and medical info on female and male infertility, rising assisted reproductive options and evolving controversies in reproductive drugs. a powerful panel of individuals offers state of the art details in a transparent and well-balanced demeanour. quantity four discusses scorching issues in modern reproductive drugs, together with stem phone applied sciences for male infertility, the present kingdom of ovarian tissue cryopreservation and time-lapse video microscopy of embryos. The extended part on controversies permits point/counterpoint dialogue among specialists with differing critiques on themes like eSET and the use and function of supplements in IVF cycles. Created to supply an ongoing appraisal of present wisdom, the Biennial evaluate of Infertility stimulates verbal exchange among all clinicians and researchers operating to assist unravel their infertility.
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Additional resources for Biennial Review of Infertility: Volume 4
2012;44:41–55. Chen B, Wang YB, Zhang ZL, Xia WL, Wang HX, Xiang ZQ, Hu K, Han YF, Wang YX, Huang YR, Wang Z. Xeno-free culture of human spermatogonial stem cells supported by human embryonic stem cell-derived fibroblast-like cells. Asian J Androl. 2009;11(5):557–65. Kokkinaki M, Djourabtchi A, Golestaneh N. Longterm culture of human SSEA-4 positive spermatogonial stem cells (SSCs). J Stem Cell Res Ther. 2011;S2:003. Liu S, Tang Z, Xiong T, Tang W. Isolation and characterization of human spermatogonial stem cells.
We immediately translated this new knowledge to the clinic and now routinely freeze intact testicular tissue pieces for patients and believe this will maximize their fertility options in the future. 7 Building a Testis Even after disaggregation into single cell suspensions, testicular cells (including germ cells, Sertoli cells, peritubular myoid cells, and Leydig cells) have the remarkable ability to reorganize to form normal-looking seminiferous tubules when grafted under the skin of recipient mice [147– 151].
Yango and colleagues reported that recovery of SSEA4+ cells from cryopreserved fetal testicular tissue was similar to cryopreserved testicular cells, but recovery of SSEA4+ cells from cryopreserved adult testicular cells was greater than cryopreserved testicular tissue . Pacchiarotti and coworkers reported that cryopreservation of testicular tissue was comparable in most aspects to cryopreservation of a cell suspension, except the viability of cells from the cryopreserved tissue was higher.