By Massimo Sabatini, Philippe Pastoureau, FrTdTric De Ceuninck
An imperative number of up to date classical and rising concepts that promise so as to add severe wisdom to our figuring out of cartilage metabolism in health and wellbeing and illness. quantity 1: mobile and Molecular instruments describes confirmed molecular and mobile suggestions for the in vitro research of ordinary and osteoarthritic cartilage via biochemical, biomolecular, immunological, and actual techniques, with emphasis at the genetic manipulation of cells. quantity 2: constitution and In Vivo research, deals state of the art tactics for studies-at the tissue level-of turnover, constitution, and functioning of standard and diseased cartilage by way of invasive and noninvasive capability. accomplished and up to date, the 2 volumes of Cartilage and Osteoarthritis supply researchers and bench scientists with with ease reproducible protocols for brand new experiments to understand-from the mobile to the animal level-the pathophysiology of cartilage and to find molecular objectives for pharmacological intervention.
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Extra info for Cartilage and Osteoarthritis
Acta 1570, 1–8. 15. Enobakhare, B. , Bader, D. , and Lee, D. A. (1996) Quantification of sulfated 15 glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue. Anal. Biochem. 243, 189–191. 16. , et al. (1994) Reexpression of car16 tilage-specific genes by dedifferentiated human chondrocytes cultured in alginate beads. Exp. Cell Res. 212, 97–104. 17. , and Adolphe, M. (1998) 17 Dedifferentiated chondrocytes cultured in alginate beads: restoration of the differentiated phenotype and of the metabolic responses to interleukin-1β.
Cytochem. 49, 1211–1219. 13. , Gunther, K. , and Brenner, R. (2002) 13 Lipofection of rabbit chondrocytes and long lasting expression of a lacZ reporter system in alginate beads. Osteoarthritis Cartilage 10, 212–217. 14. Knight, M. , Lee, D. , van Osch, G. , and Bader, D. L. (2002) Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus. Biochim. Biophys. Acta 1570, 1–8. 15. Enobakhare, B. , Bader, D. , and Lee, D. A. (1996) Quantification of sulfated 15 glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue.
Anal. Biochem. 243, 189–191. 16. , et al. (1994) Reexpression of car16 tilage-specific genes by dedifferentiated human chondrocytes cultured in alginate beads. Exp. Cell Res. 212, 97–104. 17. , and Adolphe, M. (1998) 17 Dedifferentiated chondrocytes cultured in alginate beads: restoration of the differentiated phenotype and of the metabolic responses to interleukin-1β. J. Cell. Physiol. 176, 303–313. 18. , Lee, Y. , and Dean, M. F. (1998) Re-expression of differentiated proteoglycan phenotype by dedifferentiated human chondrocytes during culture in alginate beads.