By Justin Jang Hann Chu, Swee Kim Ang
This quantity covers multidisciplinary techniques on a variety of features of Chikungunya Virus (CHIKV) examine that was once introduced jointly from top laboratories around the globe. themes coated comprise concepts in medical and diagnostic virology, easy protocols in telephone and virus tradition, bioinformatics, and proteomics ways in mobile reaction reviews. Chikungunya Virus: equipment and Protocols additionally discusses equipment in immunology and animal version experiences, in addition to diverse recommendations of antivirals and vaccine improvement for therapeutics opposed to CHIKV an infection. Written within the hugely successful Methods in Molecular Biology sequence structure, chapters contain introductions to their respective subject matters, lists of the mandatory fabric and reagents, step by step, comfortably reproducible laboratory protocols, and tips about troubleshooting and fending off identified pitfalls.
Thorough and functional, Chikungunya Virus: tools and Protocols is an invaluable and finished source for CHIKV researchers and somebody attracted to studying extra concerning the Chikungunya Virus.
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Additional resources for Chikungunya Virus: Methods and Protocols
2. 2. Double-click FigTree application icon. 3. Load the MCC tree file using the “File -> Open” path. 4. Annotate the tree that opens in the right panel as required using options available in the left panel (see Note 19). 5. Add the time scale of the tree by clicking “Scale Axis” (see Note 20). 6. Save the final tree either in PDF format or as an image file using the “File -> Export” path (Fig. 2). Tracking Evolution and Spread of Chikungunya Virus 31 Fig. 2 Time-scaled maximum clade credibility tree of East, Central, and South African genotype of CHIKV.
IEF Focusing tray with lid (same size as IPG strips). 7. Electrode wicks, precut. 8. Blotting filter papers. 9. Mineral oil. 10. Forceps, pipette for volume ranging from 4 to 1000 μl. 11. Strip plate. 12. Plastic wrap. 13. 8–16 % SDS-PAGE gels. 14. SDS-PAGE electrophoresis cell, power supply appropriate for SDS-PAGE system. 15. Tank buffer. 16. SDS-PAGE protein stain. 17. Destaining solution (40 % methanol, 10 % acetic acid). 18. Disposable rehydration/equilibration trays with lid (same size as IPG strips).
5 Construction of a Median Joining Evolutionary Network Traditionally, the most common way to present evolutionary relationships among homologous sequences is by constructing a phylogenetic tree in the form of a bifurcating tree structure. The phylogenetic tree construction generally assumes that evolution proceeds in a simplistic tree-like fashion. However, the method may not adequately represent the evolutionary history of a population in which events such as recombination, convergent evolution, and microevolution may have taken place.