Download Electric Field-Induced Effects on Neuronal Cell Biology by Dr. Tjitske Heida (auth.) PDF

By Dr. Tjitske Heida (auth.)

The idea of the classy neuron probe used to be precipitated via the prospective selective stimulation of nerves for sensible restoration after a neural lesion or sickness. The probe comprises a micro-electrode array on best of which teams of neuronal cells are cultured. an effective way to place teams of neuronal cells on most sensible of the stimulation websites of the micro-electrode array is constructed. With unfavorable dielectrophoretic forces, produced through non-uniform electrical fields on polarizable debris, neuronal cells are trapped. Experimental effects and version simulations describe the trapping strategy and its impression on neuronal telephone viability.

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Fig. 13 A Local heating of the medium due to the electric field created by the quadrupole electrode structure with triangular shaped tips. The maximum temperature rise was induced in the center of the structure just above the substrate. B The fluid flow in the xy plane just above the substrate (z=5 mm). 007 pN in this plane. C The fluid flow in the yz-plane at x=0; the length of the arrows indicates the relative velocity (Heida et al. 2002a) – Room temperature (293 K) at the vertical sides of the model and the bottom of the substrate – Heat transfer coefficient of 50 at the upper surface of the medium (representing the convection between medium and air; Incropera and de Witt 1990) – Zero velocity in z direction at the upper surface of the medium – Zero velocity in x, y, and z directions at the medium/glass interfaces (no-slip “walls”) Figure 13 shows the temperature rise in the medium in the xy plane just above the substrate and in the yz plane at x=0 (Heida et al.

From this graph no significant difference in the length of the processes over time between the two types of cells, trapped and reference, could be detected. The maximum length measured was around 30 mm for both types after 5 days in vitro. 51 Fig. 31 A Average area taken up by the soma of nonoutgrowing cortical cells during the experiment for both situations (DEP and ref). , directly after electric field application. The size of the cells were comparable for both situations, meaning that no (temporary) membrane breakdown resulting in osmotic events or changes in membrane properties had occurred.

The situation after 30 min of field application). A No input signal (reference experiment), B 1 Vpp/12 MHz, C 3 Vpp/12 MHz, D 5 Vpp/12 MHz In the absence of a field the neurons precipitated uniformly onto the surface. At 1 Vpp a uniform positioning of neurons was also obtained (image B). Neurons were trapped in the center of the electrode structure when the amplitude was increased to 3 Vpp (image C). In the lanes between the parallel sides of the electrode tips some neurons were also trapped at 3 Vpp.

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