By T. Bergan (Eds.)
The editors (of the Planta Piloto de Procesos Industriales Microbiologicos-Consejo Nacional de Investigaciones Cientificas y Tecnicas, Argentina) current 38 contributions describing step by step protocols for investigating the interactions of microorganisms with their setting. The protocols stick to an analogous structure as different volumes within the sequence, together with laboratory directions, a precis of the primary of the approach, lists of apparatus and reagents, and discussions of pitfalls. The protocols are grouped into sections on groups and biofilms; fermented milks; nucleic acids, restoration, and resolution; and experiences. evaluation articles speak about the endophytic bacterium Bacillus mojavensis, the engineering of micro organism to reinforce bioremediation of fragrant compounds, and using chemical shift reagents and Na-NMR to check sodium gradients in microorganisms.
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Extra resources for Environmental Microbiology: Methods and Protocols
Sample text
Add 10 mL of 1 mg/mL lysozyme and incubate at 37rC for 30 min. 4. Add 20 mL of 25% SDS and lyse cells using three freeze/thaw cycles (30 min at –80rC followed by 30 min at 65rC). 5. Add 60 mL of 5 M NaClO4 and 300 mL of CHCl3:isoamyl alcohol, then gently mix on a tube roller for 30 min. 6. Centrifuge in a microfuge at maximum speed, room temperature, for 5 min. 16 Greene and Voordouw 7. 5-mL Eppendorf tube and add 1 mL of 95% ethanol; incubate at –20rC for at least 1 h. Centrifuge in a microfuge at maximum speed, 4rC, for 15 min.
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Appl. Environ. Microbiol. 66, 2943–2950. 11. Flynn, S. , Loffler, F. , and Tiedje, J. M. (2000) Microbial community changes associated with a shift from reductive dechlorination of PCE to reductive dechlorination of cis-DCE and VC. Environ. Sci. Technol. 34, 1056–1061. 12. Garland, J. , Cook, K. , Adams, J. , and Kerkhof, L. (2001) Culturability as an indicator of succession in microbial communities. Microb. Ecol. 42, 150–158. 34 Liesack and Dunfield 13. , and Mattiasson, B. (2001) Biomonitoring of continuous microbial community adaptation towards more efficient phenol-degradation in a fed-batch bioreactor.